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This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference [2]) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening. This document is applicable to — products intended for human consumption, — products intended for animal feeding, — environmental samples in the area of food and feed production, handling, and — samples from the primary production stage.

This document applies to olfactory board games, cosmetic kits, gustative games and supplementary sets. It specifies requirements on the use of substances and mixtures and in some cases on their amount and concentration in olfactory board games, cosmetic kits, gustative games and supplementary sets to such games or kits.

These substances and mixtures are:

- those classified as hazardous by the EC-legislation applying to hazardous substances [13] and hazardous mixtures [13];

- substances and mixtures which in excessive amounts could harm the health of the children using them and which are not classified as hazardous by the above-mentioned legislation; and

- any other chemical substance(s) and mixture(s) delivered with the set. Furthermore, this document specifies allergenic fragrances which are prohibited in toys, marking requirements, in particular regarding allergenic fragrances, and requirements on a contents list, instructions for use, the equipment intended to be used during the activity and the use of highly flammable liquids.

This document does not apply to cosmetic toys such as play cosmetics for dolls. NOTE The terms "substance" and "mixture" are defined in the REACH regulation (EC) No. 1907/2006 [14] and in the CLP regulation (EC) No. 1272/2008 [13].

Specifies a method for the determination of the bending length of nonwovens. Gives an equation for calculating the flexural rigity of the fabric from the bending length. Does not apply to composites and laminates.

This document specifies a method of determining the attenuation variation of an optical cable during mechanical bending under load at the maximum and minimum operating temperatures.

This document specifies performance test procedures to determine the passive safety properties of support structures such as lighting columns, sign posts, signal supports, structural elements, foundations, detachable products and any other components used to support a particular item of equipment on the roadside. This document provides a common basis for the vehicle impact testing of items of road equipment support structures. This document does not apply to road restraint systems.

This document defines the design, safety and operation characteristics of gaseous hydrogen land vehicle (GHLV) refuelling connectors. GHLV refuelling connectors consist of the following components, as applicable: — receptacle and protective cap (mounted on vehicle); — nozzle; — communication hardware. This document is applicable to refuelling connectors which have nominal working pressures or hydrogen service levels up to 70 MPa. This document is not applicable to refuelling connectors dispensing blends of hydrogen with natural gas.

This document provides a description of anthropometric measurements for the head and face that can be used as a basis for creating physical and digital anthropometric databases. The list of measurements specified in this document is intended to help practitioners in the field of clothing who need to apply their knowledge to select population market segments and to create size and shape profiles for the development of all headwear garment types that extend from the torso and shoulder to the neck and head and their equivalent fitness.

The list provides guidance for taking anthropometric measurements and gives information on the principles of measurement and their underlying anatomical and anthropometrical bases relevant to clothing product development teams and head and face wear manufacturers.

ISO 4266-5 gives guidance on the selection, accuracy, installation, commissioning, calibration and verification of automatic tank thermometers (ATTs) in fiscal/custody transfer applications in which the ATT is used for measuring the temperature of petroleum and liquid petroleum products having a Reid vapour pressure less than 100 kPa, stored in cargo tanks on board marine vessels.

ISO 4266-5 is not applicable to the measurement of temperature in refrigerated storage tanks, or pressurized cargo tanks on board marine vessels.

This document specifies test methods applicable to prestressing steel (bar, wire or strand) for concrete. This document does not cover the sampling conditions that are dealt with in the product standards.

Describes test methods and requirements for the design of electrical devices to be used on small craft so that they may be operated in an explosive atmosphere without igniting surrounding flammable gases.

This document specifies test methods for slate, carbonate slate, schist and schistose stone for discontinuous roofing and external cladding, as defined in 3.1, 3.2, and 3.3 of EN 12326-1:2014 and 3.1 of prEN 12326-3:2021, used for assembly into discontinuous roofs and external wall cladding. In this sense, when the test describes a slate, it could also be considered as carbonate slate.

This document specifies the method of energy consumption measurement for the following types of industrial trucks as defined in ISO 5053‑1: — counterbalance lift truck; — articulated counterbalance lift truck; — reach truck (with retractable mast or fork arm carriage); — straddle truck; — pallet-stacking truck; — pallet truck; — platform and stillage truck; — pallet truck end controlled; — order-picking truck; — centre-controlled order-picking truck; — towing, pushing tractor and burden carrier; — towing and stacking tractor; — side-loading truck (one side only); — lateral-stacking truck (both sides); — lateral-stacking truck (three sides); — multi-directional lift truck.

This document specifies the method of energy consumption measurement for container handling lift trucks, as defined in ISO 5053‑1.

This document specifies the methods of energy consumption measurement for stacking high-lift straddle carrier (hereafter referred to as straddle carrier), as defined in ISO 5053-1:2020, 3.19.

This document specifies the main characteristics and associated test methods for assessing of polystyrene (PS) recyclates intended for use in the production of semi-finished/finished products. It is intended to support parties involved in the use of PS recyclates to agree on specifications for specific and generic applications. This document does not cover the characterization of plastics wastes, which is covered by the EN 15347 series, neither traceability topics which are covered by EN 15343.

This document specifies the main characteristics and associated test methods for assessing of polyethylene (PE) recyclates intended for use in the production of semi-finished or finished products. It is intended to support parties involved in the use of PE recyclates to agree on specifications for specific and generic applications. This document does not cover the characterization of plastics wastes, which is covered by the EN 15347 series, neither traceability topics which are covered by EN 15343.

This document specifies the main characteristics and associated test methods for assessing of polypropylene (PP) recyclates intended for use in the production of semi-finished/finished products. It is intended to support parties involved in the use of PP recyclates to agree on specifications for specific and generic applications. This document does not cover the characterization of plastics wastes, which is covered by the EN 15347 series, neither traceability topics which are covered by EN 15343.

This document is applicable to pure cultures of STEC. The present standard describes the characterization of STEC strains, isolated from any source. In particular, the characterization of STEC strains described here regard: - subtyping of the Stx-coding genes; - determination of the presence of eae gene; - determination of the presence of aggR gene; - identification of the presence of genes associated to the following serogroups: O157, O26, O111, O103, O145, O121 and O45. The full characterization of the isolated STEC strains is achieved by performing all the modules described here. The characterization scheme is not sequential, and the different modules can be implemented separately based on specific needs (e.g. Regulatory needs, Competent Authority’s requests, clients’ request). Alternative methods may be used, including Whole Genome Sequencing (WGS), provided these are verified according to the reference standard ISO 16140-2.

This document specifies a method of testing the laser-induced ignition and damage of medical beam delivery systems to allow checking of suitable products according to the classification system. NOTE 1 Take care when interpreting these results, since the direct applicability of the results of this test method to the clinical situation has not been fully established. NOTE 2 Users of products tested by this method are cautioned that the laser will be wavelength sensitive and tested at the wavelength for which it is intended to be used. If tested using other wavelengths, the power settings and modes of beam delivery need to be explicitly stated. CAUTION — This test method can involve hazardous materials, operations and equipment. This document provides advice on minimizing some of the risks associated with its use but does not purport to address all such risks. It is the responsibility of the user of this document to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use.

This document specifies a real-time PCR procedure for the quantification of the amount of roe deer DNA relative to total mammalian and poultry DNA in meat and meat products. The results of this assay for roe deer are expressed in terms of roe deer (Capreolus capreolus) haploid genome copy numbers relative to mammalian total haploid genome copy numbers. The content of roe deer can also be expressed as a percentage by mass using gravimetrically prepared calibration material from meat mixtures or model samples. The method has been previously validated in a collaborative trial and applied to DNA extracted from samples that consist of raw roe deer meat in a raw pig meat background as well as raw and boiled sausages. The limit of detection of the roe deer PCR has been determined experimentally to be at least 5 target gene copies or 0,03 % roe deer. The compliance assessment process is not part of this document.