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Ämnesområden: Livsmedelsmikrobiologi
Kommittébeteckning: SIS/TK 435/AG 05 (Livsmedelsanalyser)
Källa: CEN
Svarsdatum: den 8 mar 2024
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This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4[5],12:i:- ) lacking, or not expressing, the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression.

The method is applicable for:

— differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella non-Typhimurium serovar that has the same antigenic formula;

— identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium.

This document is applicable for the analysis of a pure culture belonging to the genus Salmonella, isolated from:

— products intended for human consumption;

— products intended for animal feeding;

— environmental samples in the area of food and feed production and handling;

— samples from the primary production stage.

This document can also be applied in other domains for identification of monophasic Salmonella Typhimurium (e.g. environmental, human health, animal health).

Ämnesområden: Livsmedelsmikrobiologi
Kommittébeteckning: SIS/TK 435/AG 05 (Livsmedelsanalyser)
Källa: CEN
Svarsdatum: den 26 apr 2024
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This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference [2]) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening. This document is applicable to — products intended for human consumption, — products intended for animal feeding, — environmental samples in the area of food and feed production, handling, and — samples from the primary production stage.

Ämnesområden: Livsmedelsmikrobiologi
Kommittébeteckning: SIS/TK 435/AG 05 (Livsmedelsanalyser)
Källa: CEN
Svarsdatum: den 3 maj 2024
Se merSe mindre
 

This document is applicable to pure cultures of STEC. The present standard describes the characterization of STEC strains, isolated from any source. In particular, the characterization of STEC strains described here regard: - subtyping of the Stx-coding genes; - determination of the presence of eae gene; - determination of the presence of aggR gene; - identification of the presence of genes associated to the following serogroups: O157, O26, O111, O103, O145, O121 and O45. The full characterization of the isolated STEC strains is achieved by performing all the modules described here. The characterization scheme is not sequential, and the different modules can be implemented separately based on specific needs (e.g. Regulatory needs, Competent Authority’s requests, clients’ request). Alternative methods may be used, including Whole Genome Sequencing (WGS), provided these are verified according to the reference standard ISO 16140-2.