Livsmedelsmikrobiologi

Ämnesområden: Livsmedelsmikrobiologi
Kommittébeteckning: SIS/TK 435/AG 05 (Livsmedelsanalyser)
Källa: CEN
Svarsdatum: den 8 mar 2024
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This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4[5],12:i:- ) lacking, or not expressing, the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression.

The method is applicable for:

— differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella non-Typhimurium serovar that has the same antigenic formula;

— identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium.

This document is applicable for the analysis of a pure culture belonging to the genus Salmonella, isolated from:

— products intended for human consumption;

— products intended for animal feeding;

— environmental samples in the area of food and feed production and handling;

— samples from the primary production stage.

This document can also be applied in other domains for identification of monophasic Salmonella Typhimurium (e.g. environmental, human health, animal health).