Livsmedelsteknik

This document specifies a procedure that can be used to determine the HADH activity in poultry meat samples by spectrophotometry. The results can provide an indication whether poultry breast meat has been previously frozen based on the ratio of relative HADH activity, and can be used to verify the labelling of poultry breast meat sold as chilled poultry. When meat is frozen and thawed, the muscle mitochondria are damaged and the HADH enzyme is released into the intracellular fluid. The relative increase in a result above a value of 0,5 for the amount ratio of HADH found in fluid pressed activity from a sample before and after laboratory freezing can be used to indicate whether it freezing, indicates that the sample has been previously frozen. The HADH activity is determined using a spectrophotometric procedure. This protocol document is applicable specifically to chicken and turkey breast meat but can be used for other cuts and/or species with appropriate limit values. Additional validation can be required. The method document is not applicable to minced meat or to poultry preparations. The compliance assessment process is not part of this document.

This part of ISO 12966 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C4 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters. The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources, and fats extracted from foodstuff. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966. This part of ISO 12966 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils. A method for the determination of the composition of fatty acid methyl esters expressed by area % in liquid vegetable oils is proposed in Annex E.