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This document describes a method for the determination of inorganic arsenic in algae by anion-exchange HPLC-ICP-MS following water bath extraction. Inorganic arsenic consists of arsenite, As(III) and arsenate, As(V). A representative test portion of the sample is treated with a diluted nitric acid and hydrogen peroxide solution in a heated water bath. Hereby the arsenic species are extracted into solution and As(III) is oxidised to As(V). The inorganic arsenic is selectively separated from other arsenic compounds using anion exchange HPLC (High Performance Liquid Chromatography) coupled on-line to the element-specific detector ICP-MS (Inductively Coupled Plasma Mass Spectrometry) for the determination of the mass fraction of inorganic arsenic. External calibration with solvent matrix-matched standards is used for quantification of the amount of inorganic arsenic. The method is based on method EN16802: Inorganic arsenic in food of plant and marine origin by HPLC-ICPMS, but covers more algae species. The present AsSugar species in certain algae can cause As peaks which might overlap with the As peaks related to the inorganic As. The current method includes a gradient elution method with quality criteria to ensure a correct identification of the inorganic arsenic.

This document describes a method for determining the amino acid profile of algal biomass. It specifies a method for the determination, in one single analysis, of the following amino acids: alanine, arginine, aspartic acid (combined with asparagine), cystine (dimer of cysteine, combined with cysteine), glutamic acid (combined with glutamine), glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and valine. This method does not apply to the determination of tryptophan. The existing draft standard ISO/DIS 4214 – Milk and milk products – Determination of amino acids in infant formula and other dairy products will be evaluated and adapted.

This document describes a method for determining the total protein content of algal biomass. Therefore, an existing method for measurement and calculation will be adapted. The method consists of the measurement of the nitrogen content by a practical test method and the calculation of the protein content by a coefficient. The document will describe the test method for nitrogen measurement. As the coefficient usually used for protein determination (6.25) is too high for algae, the document will give a recommendation for a coefficient which is more specific to algae and thereby more accurate.

This European Standard specifies requirements for performance and associated test methods for single-user anchor devices which are not permanently secured to the structure. These anchor devices incorporate stationary or travelling (mobile) anchor points designed for the attachment of components of a personal fall protection system in accordance with EN 363:2018. This European Standard also gives requirements for marking and instructions for use, and guidance on installation. This European Standard is not applicable to: - anchor devices intended to allow more than one user to be attached at any one time; - anchor devices used in any sports or recreational activity; - equipment designed to conform to EN 516:2006; - permanent anchor devices and roof safety hooks conforming to EN 17235; - elements or parts of structures which were installed for use other than as anchor points or anchor devices, e.g., beams, girders; - structural anchors (see 3.3).

This document specifies a method for measuring workers’ exposure to noise in a working environment and calculating the noise exposure level. This document deals with A-weighted levels but is applicable also to C-weighted levels. Three different strategies for measurement are specified. The method is applicable for detailed noise exposure studies or epidemiological studies of hearing damage or other adverse effects.

The measuring process requires observation and analysis of the noise exposure conditions so that the quality of the measurements can be controlled. This document provides methods for estimating the uncertainty of the results.

This document is not intended for assessment of masking of oral communication or assessment of infrasound, ultrasound and non-auditory effects of noise. It does not apply to the measurement of the noise exposure of the ear when hearing protectors are worn.

Results of the measurements performed in accordance with this document can provide useful information when defining priorities for noise control measures.

This document establishes the general principles for determining the fire resistance of various elements of construction when subjected to standard fire exposure conditions. Alternative and additional procedures to meet special requirements are given in EN 1363-2. The principle that has been embodied within all European standards relating to fire resistance testing is that where aspects and procedures of testing are common to all specific test methods e.g. the temperature/time curve, then they are specified in this test method. Where a general principle is common to many specific test methods but the details vary according to the element being tested (e.g. the measurement of unexposed face temperature), then the principle is given in this document, but the details are given in the specific test method. Where certain aspects of testing are unique to a particular specific test method (e.g. the air leakage test for fire dampers), then no details are included in this document. The test results obtained might be directly applicable to other similar elements, or variations of the element tested. The extent to which this application is permitted depends upon the field of direct application of the test result. This is restricted by the provision of rules which limit the variation from the tested specimen without further evaluation. The rules for determining the permitted variations are given in each specific test method. Variations outside those permitted by direct application are covered under extended application of test results. This results from an in-depth review of the design and performance of a particular product in test(s) by a recognized authority. Further consideration on direct and extended application is given in Annex A. The duration for which the tested element, as modified by its direct or extended field of application, satisfies specific criteria will permit subsequent classification. All values given in this document are nominal unless otherwise specified.

This document specifies a method for determining the fire resistance of air transfer grilles (ATG). It is applicable to air transfer grilles intended for installation in building components (typically walls, floors or ceilings). The orientation of the installation of the air transfer grille can be vertical or horizontal. The closing mechanism of the air transfer grille can come from expansion of material and/or from any mechanical or electrical closing device. This test method is valid for fire resistant or fire resistant and smoke control air transfer grilles. An additional test configuration is valid for fire resistant or fire resistant and smoke control air transfer grilles in applications where flame impingement is a risk during open state from start of fire (Annex A). This test method evaluates the behaviour of the air transfer grille when exposed to the standard fire curve described in EN 1363 1 and the standard pressure described in EN 1363 1. It is not the intention of this test to provide quantitative information on the rate of leakage of smoke and/or hot gases or on the transmission or generation of fumes under fire conditions. Such phenomena are only noted in describing the general behaviour of test specimens during the test. The rate of leakage of smoke at ambient temperature or at 200 °C as an optional requirement for ATG with declared smoke control will be confirmed in accordance with EN 1634 3. This test method is not valid for determining the fire resistance of air transfer grilles that are used in ducts because ATG are considered as separating elements. The test method for ATG, used in ducts is described in the corresponding duct standards. This test method is not valid for determining the fire resistance of a fire damper or a fire barrier connected to a duct on either or both sides because an ATG is tested as a fire-separating element on its own. Fire dampers are tested according to EN 1366 2. Non-mechanical fire barriers are tested according to EN 1366 12. This test method is not valid for determining the fire resistance of air transfer grilles in fire doors, shutters and openable windows as specified in EN 1634 1 and EN 1364 2, because the deformation of fire doors, shutters and openable windows in fire conditions differs from the deformation of flexible/rigid walls. Moreover, the location of thermocouples in the door standard is too specific to be handled in this document. All values given in this document are nominal unless otherwise specified.

ISO 1996-2:2017 describes how sound pressure levels intended as a basis for assessing environmental noise limits or comparison of scenarios in spatial studies can be determined. Determination can be done by direct measurement and by extrapolation of measurement results by means of calculation. This document is primarily intended to be used outdoors but some guidance is given for indoor measurements as well. It is flexible and to a large extent, the user determines the measurement effort and, accordingly, the measurement uncertainty, which is determined and reported in each case. Thus, no limits for allowable maximum uncertainty are set up. Often, the measurement results are combined with calculations to correct for reference operating or propagation conditions different from those during the actual measurement. This document can be applied on all kinds of environmental noise sources, such as road and rail traffic noise, aircraft noise and industrial noise.

This document specifies a method for sampling and handling earthworms from field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). This document applies to all terrestrial biotopes in which earthworms occur. The sampling design of field studies in general is given in ISO 18400‑101 and guidance on the determination of effects of pollutants on earthworms in field situations is given in ISO 11268‑3. These aspects can vary according to the national requirements or the climatic/regional conditions of the site to be sampled (see also Annex C). This document is not applicable for semi-terrestrial soils and it can be difficult to use under extreme climatic or geographical conditions (e.g. in high mountains). Methods for some other soil organism groups, such as collembolans, are covered in other parts of ISO 23611.

This document specifies a laboratory method for the quantification of phycocyanin content in the genus Arthrospira (Spirulina) by a spectrophotometric method.

This European standard specifies a laboratory method for the determination of the carotenoid content in microalgae. The method is based on the development of the chlorophyll a standard, which resulted in prEN 18034. This method has been validated for the microalgae species Nannochloropsis and Phaodactlylum. This standard is only validated for fucoxanthin, beta carotene and lutein content, but could be used for other carotenoids as well. Given small adaptations to be able to measure smaller concentrations, this method could also be used for macro algae. This standard is developed in CEN/TC 454/WG 6 Product test methods.

This document specifies a method for the quantitative determination of total uronic acids by High-Performance Anion Exchange Chromatography coupled with Pulsed Amperometric Detection (HPAEC-PAD) after acid hydrolysis of the samples. It provides a single analysis method for determining mannuronic, glucuronic, and guluronic acids in brown seaweed and alginate products.

This document specifies safety requirements, performance requirements, test methods, instructions for use and maintenance, and marking requirements for portable monitor assemblies. NOTE 1 Additional requirements for water nozzles and foam devices can be found in EN 15767-2 and EN 15767-3 respectively. NOTE 2 Fitting systems are dealt with in national standards or requirements, respectively. This document is applicable to portable monitor assemblies that can be both permanently installed (e.g. on a flange, a vehicle, a fire boat, etc.) and can also be used as portable monitor assemblies. This document can be read in conjunction with either part 2 or 3. This document is not applicable to monitors permanently installed on firefighting and rescue service vehicles, for which requirements are given in EN 1846-3 [2]. This document is not applicable to portable monitor assemblies which are manufactured before its date of publication.

In addition to the requirements given in EN 15767-1, this document is applicable to manual water nozzles, including water with fire extinguishing additives. It specifies requirements for safety, performance, classification and designation, as well as test methods, instructions for use and maintenance and marking. This document is not applicable to water nozzles that are manufactured before its date of publication.

In addition to the requirements given in EN 15767-1, this document applies to devices designed for aspirating air and projecting low expansion foam and, in some cases, inducting foam concentrate. It specifies requirements for safety, performance, classification and designation, as well as test methods, instructions for use and maintenance and marking. This document is not applicable to water nozzles that are manufactured before its date of publication.

This document defines the principles and specifies the requirements and guidelines for unique product identifiers, unique economic operator identifiers, and unique facility identifiers used in digital product passports. It covers the following areas: a) global uniqueness; b) persistence; c) syntax; d) semantics; e) interoperability; f) openness. This document accommodates unique product identifiers at three granularity levels of specificity: model, batch, or individual item, to support various operational needs. This document describes identification (ID) schemes that use issuing agencies, self-issuing systems, or a combination of both.

This document specifies requirements for decentralized data storage, archiving and data persistence of digital product passports. The archiving service securely stores historical passport data, preserving a comprehensive record of past information. This feature is particularly relevant for market surveillance purposes. Persistence is required to make sure that data included in the digital product passports remains available even when the economic operator creating the digital product passport is no longer active. This document also specifies requirements for the replication between economic operators and back-up operators as well as rules for data lifetime definition.

This document aims to standardize the specifications for the API of the Digital Product Passport (DPP) as mandated by the ESPR of the European Commission. The purpose of this API is to facilitate the searchability of DPPs, as well as to provide the necessary means for interactions throughout the lifecycle of a product's DPP.

The scope of this document includes: — the semantic description of a product, including its properties where relevant and the semantic aspects to represent the product lifecycle; — a common information model allowing for the implementation of data dictionary systems; — metadata models and formats to be used in exchange and representation, allowing for the integration of dictionaries; — rules on how to systematically use such metadata models when developing product group specific data models and dictionaries; — technical and organizational interoperability. This document follows the approach of standard interoperability layers and proposes the following aspects in this regard.