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This document provides standard procedures for the collection, handling and storage of soil for subsequent biological testing under aerobic conditions in the laboratory. It applies to the collection, handling and storage for assessing the effects of soil on microorganisms, invertebrates (e.g. survival, reproduction, growth, behaviour) and plants (e.g. development, growth). This document is not applicable to the handling of soil where anaerobic conditions need to be maintained throughout.
This document describes how to minimize the effects of differences in temperature, water content, and availability of oxygen on aerobic processes as well as the fractionation of soil particles to facilitate reproducible laboratory determinations[1][2].
This document is mainly applicable to temperate soils. Soils collected from extreme climates (e.g. permafrost, tropical soils) can require special handling.
NOTE This document does not provide standard procedures on the collection, handling and storage of soil organisms when assessing the structure and function of soil organism communities in the field. Such standard procedures are provided in ISO 23611‑1 to ISO 23611‑6.
This document specifies two methods for the determination of total organic carbon (TOC) in sludge, treated biowaste, soil and waste samples containing more than 0,1 % carbon in relation to the dry matter (dm).
This document is validated for several types of matrices as indicated in Table 1 (see also Annex A for the results of the validation). The results in this document are expressed in % C in relation to the dry matter (dm).
Table 1 — Matrices for which this document is applicable and validated for
NOTE Sludge amended soil, Agricultural soil Filter cake, Bottom ash, Electro-plating sludge, Dredged sludge, Rubble This method can also be applied to other environmental solid matrices, provided the user has verified the applicability.
WARNING — Persons using this document should be familiar with usual laboratory practice. This document does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices.
IMPORTANT — It is absolutely essential that tests conducted according to this document be carried out by suitably trained staff.