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This document is one of a family of International Standards providing guidance on soils and soil materials in relation to certain functions and uses including conservation of biodiversity. It applies in conjunction with these other standards. It provides guidance on the selection of experimental methods for the assessment of the ecotoxic potential of soils and soil materials (e.g. excavated and remediated soils, refills, embankments) with respect to their intended use and possible adverse effects on aquatic and soil dwelling organisms.
NOTE This is a reflection of the maintenance of the habitat and retention function of the soil. In fact, the methods listed in this document are suitable for usage in a TRIAD approach, i.e. for an ecological assessment of potentially contaminated soils (see Reference [18]).
This document does not cover tests for bioaccumulation.
The ecological assessment of uncontaminated soils with a view to natural, agricultural or horticultural use is not within the scope of this document. Such soils can be of interest if they can serve as a reference for the assessment of soils from contaminated sites (see References [29], [30] and [33]).
The interpretation of results gained by applying the proposed methods is not in the scope of this document.
This document specifies a chronic test method for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Hypoaspis (Gaeolaelaps) aculeifer. This method is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites under concern and waste materials (e.g. dredged material, municipal sludge from a wastewater treatment plant, composed material, or manure, especially those for possible land disposal). The reproduction (= number of juveniles) is the measured parameter of the test. The test reflects the bioavailability of a mixture of contaminants in natural soils (contaminated site soils) to a species which represents a trophic level which is not covered by other ISO standards. This test is not intended to replace the earthworm (see ISO 11268-2[2]) or Collembola (see ISO 11267[1]) reproduction tests since this species belongs not only to a different trophic group but also a different taxonomic group (= mites; i.e. arachnids) than those used usually.
Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil. Depending on the objective of the study, the control and dilution substrate (dilution series of contaminated soil or waste material) are either an uncontaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g. artificial soil).
This document provides information on how to use this method for testing samples (soils or substances) under temperate conditions.
This document is not applicable to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa at 25 °C.
NOTE The stability of the test substance cannot be ensured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.
This document specifies the procedure for determining bulk, dry and grain densities as well as open porosity of a rock specimen using the immersion method and a combination of the linear method and gas pycnometry.
This document is applicable to the laboratory determination of density and open porosity of a rock test specimen within the scope of geotechnical investigations.
Other methods for determining the bulk mass, grain mass, bulk volume and grain volume of the specimen that fulfil the requirements set in this document may also be considered acceptable (Annex B). Used method should be chosen based on the use case (type of specimen, availability etc.).
Bulk, dry and grain density as well as open porosity are determined for the specimen as is and are dependent on fluid content, which shall be determined and reported as per ISO 16383-1.
NOTE This document fulfils the requirements of the determination of bulk density of rock for geotechnical investigation and testing in accordance with EN 1997-2 (2024).
This document describes in a general way the application of the soil quality TRIAD approach for the site specific ecological risk assessment of contaminated soils. In detail, it presents in a transparent way three lines of evidence (chemistry, ecotoxicology and ecology) which together allow an efficient, ecologically robust but also practical risk assessment of contaminated soils. This procedure can also be applicable to other stress factors, such as acidification, soil compaction, salinization, loss of soil organic substance, and erosion. However, so far, no experience has been gained with these other applications. Therefore, this document focuses on soils contaminated by chemicals.
NOTE 1 This document focuses on ecological risk assessment. Thus, it does not cover human health end points.
In view of the nature of this document, the investigation procedure is described on a general level. It does not contain details of technical procedures for the actual assessment. However, this document includes references relating to technical standards (e.g. ISO 15799, ISO 17616) which are useful for the actual performance of the three lines of evidence.
In ecological risk assessment, the effects of soil contamination on the ecosystem are related to the intended land use and the requirements that this use sets for properly functioning soil. This document describes the basic steps relating to a coherent tool for a site-specific risk assessment with opportunities to work out site specific details.
This document can also be used for the evaluation of clean-up operations, remediation processes or management measures (i.e. for the evaluation of the environmental quality after having performed such actions).
NOTE 2 The application of this document starts when it has already been decided that an ecological risk assessment at a given site needs to be performed. In other words, the practical performance of the soil quality TRIAD and the evaluation of the individual test results will be described. Thus, nothing will be said about decisions whether (and if yes, how) the results of the assessment are included in soil management measures or not.
NOTE 3 The TRIAD approach can be used for different parts of the environment, but this document focuses mostly on the soil compartment. Comparable documents for other environmental compartments are intended to be prepared in addition (e.g. the terrestrial aboveground compartment) in order to perform a complete site assessment, based on the same principles and processes.