Undersökning av vattens biologiska egenskaper

Kommittébeteckning: SIS/TK 426 (Biologiska vattenundersökningar)
Källa: ISO
Svarsdatum: den 6 jul 2018
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This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as % of unexposed control and toxicity quantified based on % cell viability vs. % effluent or chemical concentration response curves (see Clause 9).