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This document describes a method for the determination of inorganic arsenic in algae by anion-exchange HPLC-ICP-MS following water bath extraction. Inorganic arsenic consists of arsenite, As(III) and arsenate, As(V). A representative test portion of the sample is treated with a diluted nitric acid and hydrogen peroxide solution in a heated water bath. Hereby the arsenic species are extracted into solution and As(III) is oxidised to As(V). The inorganic arsenic is selectively separated from other arsenic compounds using anion exchange HPLC (High Performance Liquid Chromatography) coupled on-line to the element-specific detector ICP-MS (Inductively Coupled Plasma Mass Spectrometry) for the determination of the mass fraction of inorganic arsenic. External calibration with solvent matrix-matched standards is used for quantification of the amount of inorganic arsenic. The method is based on method EN16802: Inorganic arsenic in food of plant and marine origin by HPLC-ICPMS, but covers more algae species. The present AsSugar species in certain algae can cause As peaks which might overlap with the As peaks related to the inorganic As. The current method includes a gradient elution method with quality criteria to ensure a correct identification of the inorganic arsenic.

This document describes a method for determining the amino acid profile of algal biomass. It specifies a method for the determination, in one single analysis, of the following amino acids: alanine, arginine, aspartic acid (combined with asparagine), cystine (dimer of cysteine, combined with cysteine), glutamic acid (combined with glutamine), glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and valine. This method does not apply to the determination of tryptophan. The existing draft standard ISO/DIS 4214 – Milk and milk products – Determination of amino acids in infant formula and other dairy products will be evaluated and adapted.

This document describes a method for determining the total protein content of algal biomass. Therefore, an existing method for measurement and calculation will be adapted. The method consists of the measurement of the nitrogen content by a practical test method and the calculation of the protein content by a coefficient. The document will describe the test method for nitrogen measurement. As the coefficient usually used for protein determination (6.25) is too high for algae, the document will give a recommendation for a coefficient which is more specific to algae and thereby more accurate.

This document specifies a laboratory method for the quantification of phycocyanin content in the genus Arthrospira (Spirulina) by a spectrophotometric method.

This European standard specifies a laboratory method for the determination of the carotenoid content in microalgae. The method is based on the development of the chlorophyll a standard, which resulted in prEN 18034. This method has been validated for the microalgae species Nannochloropsis and Phaodactlylum. This standard is only validated for fucoxanthin, beta carotene and lutein content, but could be used for other carotenoids as well. Given small adaptations to be able to measure smaller concentrations, this method could also be used for macro algae. This standard is developed in CEN/TC 454/WG 6 Product test methods.

This document specifies a method for the quantitative determination of total uronic acids by High-Performance Anion Exchange Chromatography coupled with Pulsed Amperometric Detection (HPAEC-PAD) after acid hydrolysis of the samples. It provides a single analysis method for determining mannuronic, glucuronic, and guluronic acids in brown seaweed and alginate products.