Generella metoder för kontroll och analys av livsmedel

Kommittébeteckning: SIS/TK 435 (Livsmedel och foder)
Källa: CEN
Svarsdatum: den 2 okt 2018
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This document describes a method for the determination of aluminium in food by inductively coupled plasma optical emission spectrometry (ICP-OES) after pressure digestion. This method is suitable for mass fraction in the range of 15 mg/kg to 200 mg/kg. At concentrations above 200 mg/kg digestion temperatures higher than 220 °C can be necessary to recover the aluminium as completely as possible.

Kommittébeteckning: SIS/TK 435 (Livsmedel och foder)
Källa: ISO
Svarsdatum: den 15 okt 2018
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This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods such as PCR including different post-PCR detection methods, real-time PCR, single and/or multiple probe-based detection techniques as well as the combination of such methods.

The document applies to the detection, identification, quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods.

Note: At the time this document was developed it concerned only mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A.

Kommittébeteckning: SIS/TK 435 (Livsmedel och foder)
Källa: CEN
Svarsdatum: den 16 okt 2018
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This document describes a screening method for the determination of aflatoxin B1, deoxynivalenol, fumonisin B1 and B2, ochratoxin A, HT-2 and T-2 toxins, and zearalenone in foodstuffs by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS).

The aim of the screening method is to test compliance of foodstuff with regulatory limits or to determine whether a certain pre-defined level (the screening target concentration, STC) is exceeded or not. The result of the screening is either “negative” or “suspect”. “Negative” (screen negative) means that the targeted mycotoxins are not detected or potentially present but below the STC. “Suspect” (screen positive) means that the established cut-off level is exceeded and the sample can contain one or more mycotoxins at a level higher than the STC.

For full identification and accurate quantification a second confirmatory quantitative analysis method is required which is outside the scope of this document.

The method is suitable for various types of foodstuff and has been validated for representative matrices from four commodity groups:

— high starch and/or protein content and low water and fat content: wheat, cereal mixture, wheat flour and cornflakes;

— high oil content: peanuts;

— high sugar low water content: figs;

— high water content: grape juice.

During validation, cut-off levels were established for the following screening target concentrations:

— aflatoxin B1: 2 μg/kg to 5 μg/kg;

— deoxynivalenol: 250 μg/kg to 865 μg/kg;

— fumonisin B1: 200 μg/kg to 790 μg/kg;

— fumonisin B2: 110 μg/kg to 230 μg/kg;

— ochratoxin A: 4 μg/kg to 9 μg/kg;

— T-2 toxin: 25 μg/kg;

— HT-2 toxin: 25 μg/kg to 50 μg/kg;

— zearalenone: 30 μg/kg to 100 μg/kg.

Kommittébeteckning: SIS/TK 435 (Livsmedel och foder)
Källa: CEN
Svarsdatum: den 16 okt 2018
Se merSe mindre
 

This document specifies a method for the determination of aluminium in food by inductively coupled plasma mass spectrometry (ICP-MS) after pressure digestion. This method is suitable for mass fractions in the range of 1 mg/kg to 200 mg/kg. At concentrations above 200 mg/kg digestion temperatures higher than 220 °C can be necessary to recover the aluminium as completely as possible.