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This document specifies a method for detection of HAV and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multicomponent foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

This document specifies a method for detection of HAV and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multicomponent foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

This document provides the overall framework for detection of sequences corresponding to species containing allergens using the polymerase chain reaction (PCR). It relates to the requirements for the specific amplification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified nucleic acid sequence.

Guidelines, minimum requirements and performance criteria laid down in this document are intended to ensure that comparable and reproducible results are obtained in different laboratories. This document has been established for food matrices.

This International Standard gives requirements and guidance for the estimation and expression of measurement uncertainty (MU) associated with quantitative results in microbiology of the food chain.

It is applicable to the quantitative analysis of:

— products intended for human consumption or the feeding of animals;

— environmental samples in the area of food production and food handling;

— samples at the stage of primary production.

The quantitative analysis is typically carried out by enumeration of microorganisms using a colony-count technique. It is also generally applicable to other quantitative analyses, including Most Probable Number (MPN) techniques and instrumental methods, such as impediometry and flow cytometry.

The uncertainty estimated by this International Standard does not include systematic effects ("trueness" or "bias").

1.1 This document specifies the general criteria for tests of sterility on medical devices that have been exposed to a treatment with the sterilizing agent reduced relative to that anticipated to be used in routine sterilization processing. These tests are intended to be performed when defining, validating or maintaining a sterilization process.

1.2 This document is not applicable to:

a) sterility testing for routine release of product that has been subjected to a sterilization process,

b) performing a test for sterility (see 3.12), and

NOTE 1 The performance of a) or b) is not a requirement of ISO 11135, ISO 11137-1, ISO 14160, ISO 14937, ISO 17665-1 or ISO 20857.

c) culturing of biological indicators or inoculated products.

NOTE 2 Guidance on culturing biological indicators is included in ISO 11138-7[5].