Naturvetenskap och tillämpad vetenskap

This part of ISO 4833 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to:
a) products intended for human consumption and for animal feed;
b) environmental samples in the area of food and feed production and handling.

This part of ISO 4833 is applicable to:
1) products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples);
2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp.

The applicability of this part of ISO 4833 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively.

For some matrices, the method specified in this part of ISO 4833 can give different results to those obtained using the method specified in ISO 4833-2.

This international standard specifies minimum requirements for generating and analyzing whole-genome sequencing (WGS) data obtained from foodborne bacteria. These requirements are applicable to any sequencing platform or chemistry. This process may include the following stages:

a) Handling of bacterial cultures;

b) Genomic DNA isolation;

c) Library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;

d) Bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;

e) Metadata capture and sequence repository deposition; and

f) Validation of the end-to-end WGS workflow (fit for purpose for intended application).